Regulation of trophoblast differentiation by the PI3-K/Akt signaling pathway

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dc.contributor.author Canham, Lindsey
dc.date.accessioned 2006-06-30T20:07:20Z
dc.date.available 2006-06-30T20:07:20Z
dc.date.issued 2006-06-30T20:07:20Z
dc.identifier.uri http://hdl.handle.net/2271/143
dc.description Developmental Biology Room G026 Dykes 11:12 AM Abstract 213 en
dc.description.abstract Trophoblast cells are essential for the establishment and maintenance of pregnancy. In rodents, trophoblast giant cells are located at the maternal-embryonic interface and represent one of the major endocrine cell types of the rodent placenta. The endocrine phenotype of trophoblast giant cells includes production of peptide and steroid hormones. In this study we evaluate the role of the PI3-K/Akt signaling pathway in regulation of the endocrine phenotype of trophoblast giant cells. This study employed the Rcho-1 trophoblast cells as an in vitro trophoblast cell model system. Rcho-1 trophoblast cells can be maintained in a stem cell state or induced to differentiate along the trophoblast giant cell lineage. Inhibition of the PI3-K/Akt signaling pathway in Rcho-1 cells leads to decreased expression of peptide hormones: placental lactogen II and prolactin-like-protein A. Other peptide hormones such as placental lactogen I are unaffected. Likewise, disruption of the PI3-K/Akt signaling pathway significantly inhibited Rcho-1 trophoblast cell production of androstenedione, as measured by radioimmunoassay. Inhibition of the PI3-K/Akt pathway also significantly interfered with the expression of genes encoding key regulatory proteins controlling androstenedione biosynthesis and metabolism, including 3-beta hydroxysteroid dehydrogenase, 17-alpha hydroxylase and 17-beta hydroxysteroid dehydrogenase. The expression of other genes encoding regulatory proteins controlling steroidogenesis (StAR and cytochrome P450 side-chain cleavage) was not significantly altered by inhibition of this pathway. In summary, the PI3-K/Akt signaling pathway participates in the regulation of the trophoblast giant cell endocrine phenotype and potentially the maintenance of pregnancy. en
dc.description.sponsorship Soares, Michael Pathology and Laboratory Medicine en
dc.format.extent 1966592 bytes
dc.format.mimetype application/vnd.ms-powerpoint
dc.language.iso en_US en
dc.subject placenta en
dc.subject hormones en
dc.title Regulation of trophoblast differentiation by the PI3-K/Akt signaling pathway en
dc.type Presentation en

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