Differential vulnerabilities of nociceptive subpopulations in diabetic neuropathy

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dc.contributor.author Johnson, Megan S
dc.date.accessioned 2006-06-30T20:10:55Z
dc.date.available 2006-06-30T20:10:55Z
dc.date.issued 2006-06-30T20:10:55Z
dc.identifier.uri http://hdl.handle.net/2271/156
dc.description Neuroscience III Room 1028 Dykes 1:42 PM Abstract 202 en
dc.description.abstract The perpetual state of hyperglycemia existing in diabetes can permanently damage peripheral nerves, resulting in diabetic neuropathy (DN). The most common long-term complication of diabetes, DN causes reductions in intraepidermal nerve fiber (IENF) density that are extensively reported in humans and diabetic animal models. Currently, most studies focus on overall fiber density using PGP9.5, a marker of all epidermal fibers, in part because visualizing markers of fiber subtypes is technically problematic. However, neuronal subpopulations can be differentially vulnerable to settings of nerve injury or disease, perhaps accounting for different sensory symptoms associated with each condition. This study was undertaken to assess whether diabetes differentially affects nerve fiber subtypes, in particular peptidergic and nonpeptidergic subpopulations. To address the inherent difficulties associated with visualizing different subpopulations at the epidermal level, we used transgenic mice expressing green fluorescent protein (GFP) from the MrgD gene promoter, the product of which is expressed only in nonpeptidergic neurons innervating the skin. Diabetes was induced in 8-week-old MrgD mice by intraperitoneal injection of streptozotocin. After 4 weeks, control and diabetic mice were sacrificed and their hindpaw footpads removed and fixed in Zamboni’s fixative. Nonpeptidergic innervation was quantified in 30μm sections by counting the number of GFP-fluorescent IENF. Using GFP fluorescence in conjunction with PGP9.5 immunostaining, peptidergic IENF density was extrapolated. Results from this study will allow us to characterize the contribution of these different subpopulations to the overall fiber loss seen in DN as well as deepen our understanding of their functional roles in nociception. en
dc.description.sponsorship Wright, Douglas E. Anatomy and Cell Biology en
dc.format.extent 16096256 bytes
dc.format.mimetype application/vnd.ms-powerpoint
dc.language.iso en_US en
dc.subject Neuropathy en
dc.subject Diabetes en
dc.subject Cutaneous innervation en
dc.title Differential vulnerabilities of nociceptive subpopulations in diabetic neuropathy en
dc.type Presentation en

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