The role of the ECM during regeneration in Hydra and Zebrafish

Abstract

After injury or amputation, the mechanisms involved in tissue regeneration are highly complex and coordinated. Certain lower invertebrates such as the Hydra and vertebrates such as zebrafish (Danio rerio) present excellent animal models to study basement membrane structure and function during regeneration under in vivo conditions. This is due to their simple body structures and capacity for regeneration. Currently, there remains much to be understood about the molecular and cellular mechanisms for regeneration in these creatures. Understanding biogenesis (synthesis, assembly, and turnover) of the basement membrane in hydra may have clinical relevance to the biogenesis and pathology in the glomerular basement membrane. We have used molecular biology and immunocytochemical approaches to investigate the synthesis and turnover of ECM molecules during regeneration. This study lays a foundation for further understanding of molecular