Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet

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dc.contributor.author Howard Hsu en_US
dc.contributor.author Nathan Culley en_US
dc.date.accessioned 2009-05-05T16:14:33Z
dc.date.available 2006 - en_US
dc.date.available 2009-05-05T16:14:33Z
dc.date.issued 2006-06-23 en_US
dc.identifier.citation Howard Hsu;Nathan Culley: Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet. Lipids Health Dis 2006, 5(1):25. en_US
dc.identifier.uri http://www.lipidworld.com/content/5/1/25 en_US
dc.identifier.uri http://hdl.handle.net/2271/599
dc.description.abstract BACKGROUND:It has been shown that calcifying vesicles play an important role in aortic calcification and that cholesterol content in the isolated vesicle fraction is increased when rabbits are fed supplemental cholesterol diets. Whether lipoprotein-associated cholesterols and other lipids are also increased in the vesicle fraction and whether the increase correlates with atherosclerosis remain unknown.RESULTS:Fourteen juvenile male rabbits fed an atherogenic diet containing 0.5% cholesterol and 2% peanut oil for 3 months developed varying degrees of hypercholesterolemia and intimal thickening in the ascending thoracic aorta. The correlation between these two parameters was insignificant, and likely attributable to the use of small numbers of rabbits in this study. Despite this lack of correlation, we demonstrate that the accumulation of cholesterol in calcifying vesicle fractions obtained from the collagenase-digested aorta fragments correlates well with intimal thickening (r2 = 0.98, p < 0.0001). To a smaller degree, the correlation was also significant between intimal thickening and the cholesterol accumulation in the microsomal and post-vesicle fractions. The cholesterol supplemental diet increased the low density lipoprotein-cholesterol (LDL-C) content in calcifying vesicle fractions by 3-fold but did not affect the triglyceride content. High density lipoprotein-cholesterol (HDL-C) and very low-density lipoprotein cholesterol (VLD-C) were absent in calcifying vesicle fractions.CONCLUSION:When limited numbers of rabbits are used, LDL-C accumulation in calcifying vesicle fractions is a better biomarker for atherosclerosis than LDL-C levels in the serum. The close association of LDL-C with calcifying vesicles may play an important role in atherosclerosis and calcification. en_US
dc.format.extent 373116 bytes
dc.format.extent 2910 bytes
dc.format.extent 12055 bytes
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dc.format.extent 43 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
dc.language en en_US
dc.language.iso en_US
dc.publisher BioMedCentral en_US
dc.relation.ispartof 1476-511X en_US
dc.relation.hasversion http://www.biomedcentral.com/content/pdf/1476-511X-5-25.pdf en_US
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Aorta, Thoracic/pathology en_US
dc.subject.mesh Aortic Diseases/ prevention & control en_US
dc.subject.mesh Arteriosclerosis/ etiology/prevention & control en_US
dc.subject.mesh Calcinosis/ etiology/prevention & control en_US
dc.subject.mesh Calcium/blood/deficiency en_US
dc.subject.mesh Calcium, Dietary/ therapeutic use en_US
dc.subject.mesh Cholesterol, Dietary/ adverse effects en_US
dc.subject.mesh Diet, Atherogenic en_US
dc.subject.mesh Jaundice/ etiology en_US
dc.subject.mesh Male en_US
dc.subject.mesh Rabbits en_US
dc.title Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet en_US
dc.type article en_US
dc.date.captured 2009-04-27 en_US
dc.identifier.doi doi:10.1186/1476-511X-5-25 en_US
dc.identifier.pmid 16796746 en_US

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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