Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis

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dc.contributor.author Howard Hsu en_US
dc.contributor.author Antonio Artigues en_US
dc.contributor.author Maria Villar en_US
dc.date.accessioned 2009-05-05T16:14:39Z
dc.date.available 2008 - en_US
dc.date.available 2009-05-05T16:14:39Z
dc.date.issued 2006-10-16 en_US
dc.identifier.citation Howard Hsu;Antonio Artigues;Maria Villar: Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis. Lipids Health Dis 2008, 7(1):2. en_US
dc.identifier.uri http://www.lipidworld.com/content/7/1/2 en_US
dc.identifier.uri http://hdl.handle.net/2271/600
dc.description.abstract BACKGROUND:Since aortic calcification has been shown to initiate in the lower zone of well-thickened plaques (LZP) adjacent to the aortic media of rabbits fed supplemental cholesterol diets, a restricted supply of serum to vascular cells could play a role in vascular calcification. This study was designed to use a cell culture model to support this hypothesis.RESULTS:Rabbit aortic smooth muscle cells were grown to confluence in a culture media containing 10 % fetal bovine serum (FBS). The confluent cells were then exposed to the media for 2 hrs with or without serum at a Ca × P ion product range of 4.5–9.4 mM2. In contrast to the cells cultured in the presence of FBS, confluent cells in its absence displayed marked mineral-positive alizarin red staining and infrared absorption of mineral phosphate. A kinetic parameter C1/2 was used to designate the concentration of serum or its protein constituents needed to reduce the deposition of Ca and P by half. The C1/2 for FBS and rabbit serum was 0.04–0.07 % The C1/2 value for rabbit serum proteins was 13.5 µg/ml corresponding to the protein concentration in 0.06 % of serum. This C1/2 was markedly smaller than 86.2 µg/ml for bovine serum albumin present in 0.37 % serum (p < 0.05). Serum depletion also caused marked membrane translocation as evidenced through a specific apoptosis dye uptake by cells. The proteomic analysis of calcifying vesicles, which can be released by serum depletion, revealed several calcification-related proteins.CONCLUSION:The aortic smooth muscle cell culture model suggests that serum depletion may play a role in the initiation of aortic calcification. The serum exhibits remarkable ability to inhibit cell-mediated calcification. en_US
dc.format.extent 1101095 bytes
dc.format.extent 2910 bytes
dc.format.extent 12055 bytes
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dc.format.extent 43 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
dc.language en en_US
dc.language.iso en_US
dc.publisher BioMedCentral en_US
dc.relation.ispartof 1476-511X en_US
dc.relation.hasversion http://www.biomedcentral.com/content/pdf/1476-511X-7-2.pdf en_US
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Aorta, Thoracic/metabolism/ pathology en_US
dc.subject.mesh Aortic Diseases/etiology/metabolism/pathology en_US
dc.subject.mesh Biological Markers/metabolism en_US
dc.subject.mesh Calcinosis/metabolism/ pathology en_US
dc.subject.mesh Cholesterol, Dietary/adverse effects/ metabolism en_US
dc.subject.mesh Cholesterol, LDL/ metabolism en_US
dc.subject.mesh Coronary Artery Disease/etiology/metabolism/pathology en_US
dc.subject.mesh Cytoplasmic Vesicles/metabolism/ pathology en_US
dc.subject.mesh Diet, Atherogenic en_US
dc.subject.mesh Dietary Supplements/adverse effects en_US
dc.subject.mesh Hypercholesterolemia/blood en_US
dc.subject.mesh Male en_US
dc.subject.mesh Microsomes/metabolism/pathology en_US
dc.subject.mesh Rabbits en_US
dc.subject.mesh Tunica Intima/metabolism/ pathology en_US
dc.title Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis en_US
dc.type article en_US
dc.date.captured 2009-04-27 en_US
dc.identifier.doi doi:10.1186/1476-511X-7-2 en_US
dc.identifier.pmid 17042957 en_US

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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