Ubp43 gene expression is required for normal Isg15 expression and fetal development

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dc.contributor.author Lea Rempel en_US
dc.contributor.author Kathleen Austin en_US
dc.contributor.author Kenneth Ritchie en_US
dc.contributor.author Ming Yan en_US
dc.contributor.author Meifeng Shen en_US
dc.contributor.author Dong-Er Zhang en_US
dc.contributor.author Luiz Henkes en_US
dc.contributor.author Thomas Hansen en_US
dc.date.accessioned 2009-05-05T16:14:58Z
dc.date.available 2007 - en_US
dc.date.available 2009-05-05T16:14:58Z
dc.date.issued 2006-10-09 en_US
dc.identifier.citation Lea Rempel;Kathleen Austin;Kenneth Ritchie;Ming Yan;Meifeng Shen;Dong-Er Zhang;Luiz Henkes;Thomas Hansen: Ubp43 gene expression is required for normal Isg15 expression and fetal development. Reproductive Biology and Endocrinology 2007, 5(1):13. en_US
dc.identifier.uri http://www.rbej.com/content/5/1/13 en_US
dc.identifier.uri http://hdl.handle.net/2271/603
dc.description.abstract BACKGROUND:Isg15 covalently modifies murine endometrial proteins in response to early pregnancy. Isg15 can also be severed from targeted proteins by a specific protease called Ubp43 (Usp18). Mice lacking Ubp43 (null) form increased conjugated Isg15 in response to interferon. The Isg15 system has not been examined in chorioallantoic placenta (CP) or mesometrial (MM) components of implantation sites beyond 9.5 days post coitum (dpc). It was hypothesized that deletion of Ubp43 would cause disregulation of Isg15 in implantation sites, and that this would affect pregnancy rates.METHODS:Heterozygous (het) Ubp43 mice were mated and MM and CP implantation sites were collected on 12.5 and 17.5 days post-coitum (dpc).RESULTS:Free and conjugated Isg15 were greater on 12.5 versus 17.5 dpc in MM. Free and conjugated Isg15 were also present in CP, but did not differ due to genotype on 12.5 dpc. However, null CP had greater free and conjugated Isg15 when compared to het/wt on 17.5 dpc. Null progeny died in utero with fetal genotype ratios (wt:het:null) of 2:5:1 on 12.5 and 2:2:1 on 17.5 dpc. Implantation sites were disrupted within the junctional zone and spongiotrophoblast, contained less vasculature based on lectin B4 staining and contained greater Isg15 mRNA and VEGF protein in Ubp43 null when compared to wt placenta.CONCLUSION:It is concluded that Isg15 and its conjugates are present in implantation sites during mid to late gestation and that deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. Also, under mixed genetic background, deletion of Ubp43 results in fetal death. en_US
dc.format.extent 527217 bytes
dc.format.extent 2910 bytes
dc.format.extent 12055 bytes
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dc.format.extent 43 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
dc.language en en_US
dc.language.iso en_US
dc.publisher BioMedCentral en_US
dc.relation.ispartof 1477-7827 en_US
dc.relation.hasversion http://www.biomedcentral.com/content/pdf/1477-7827-5-13.pdf en_US
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Female en_US
dc.subject.mesh Gene Expression Regulation/drug effects en_US
dc.subject.mesh HLA Antigens/genetics/ physiology en_US
dc.subject.mesh Histocompatibility Antigens Class I/genetics/ physiology en_US
dc.subject.mesh Humans en_US
dc.subject.mesh Immunosuppression en_US
dc.subject.mesh Maternal-Fetal Exchange/genetics/immunology/physiology en_US
dc.subject.mesh Oxygen/pharmacology en_US
dc.subject.mesh Papio/genetics en_US
dc.subject.mesh Placenta/metabolism/physiology en_US
dc.subject.mesh Pregnancy en_US
dc.subject.mesh Regulatory Sequences, Nucleic Acid en_US
dc.title Ubp43 gene expression is required for normal Isg15 expression and fetal development en_US
dc.type article en_US
dc.date.captured 2009-04-27 en_US
dc.identifier.doi doi:10.1186/1477-7827-5-13 en_US
dc.identifier.pmid 17118165 en_US

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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