Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes

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dc.contributor.author Oxana Norkina en_US
dc.contributor.author Rolf Graf en_US
dc.contributor.author Philippe Appenzeller en_US
dc.contributor.author Robert De Lisle en_US
dc.date.accessioned 2009-05-05T16:43:53Z
dc.date.available 2006 - en_US
dc.date.available 2009-05-05T16:43:53Z
dc.date.issued 2008-08-26 en_US
dc.identifier.citation Oxana Norkina;Rolf Graf;Philippe Appenzeller;Robert De Lisle: Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes. BMC Gastroenterol 2006, 6(1):16. en_US
dc.identifier.uri http://www.biomedcentral.com/1471-230X/6/16 en_US
dc.identifier.uri http://hdl.handle.net/2271/632
dc.description.abstract BACKGROUND:The cystic fibrosis (CF) mouse pancreas has constitutively elevated expression of the Reg/PAP cell stress genes (60-fold greater Reg3a, and 10-fold greater PAP/Reg3ß and Reg3?). These genes are suggested to be involved in protection or recovery from pancreatic injury.METHODS:To test this idea the supramaximal caerulein model was used to induce acute pancreatitis in wild type and CF mice. Serum amylase, pancreatic water content (as a measure of edema), pancreatic myeloperoxidase activity, and Reg/PAP expression were quantified.RESULTS:In both wild type and CF mice caerulein induced similar elevations in serum amylase (maximal at 12 h), pancreatic edema (maximal at 7 h), and pancreatic myeloperoxidase activity (MPO, a marker of neutrophil infiltration; maximal at 7 h). By immunohistochemistry, Reg3a was strongly expressed in the untreated CF pancreas but not in wild type. During pancreatitis, Reg3a was intensely expressed in foci of inflamed tissue in both wild type and CF.CONCLUSION:These data demonstrate that the severity of caerulein-induced pancreatitis is not ameliorated in the CF mouse even though the Reg/PAP stress genes are already highly upregulated. While Reg/PAP may be protective they may also have a negative effect during pancreatitis due to their anti-apoptotic activity, which has been shown to increase the severity of pancreatitis. en_US
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dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.format.mimetype application/octet-stream
dc.format.mimetype application/octet-stream
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dc.language en en_US
dc.language.iso en_US
dc.publisher BioMedCentral en_US
dc.relation.ispartof 1471-230X en_US
dc.relation.hasversion http://www.biomedcentral.com/content/pdf/1471-230X-6-16.pdf en_US
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.subject.mesh Adult en_US
dc.subject.mesh Aged en_US
dc.subject.mesh Attitude of Health Personnel en_US
dc.subject.mesh Decision Making en_US
dc.subject.mesh Factor Analysis, Statistical en_US
dc.subject.mesh Female en_US
dc.subject.mesh Humans en_US
dc.subject.mesh Hypersensitivity/ diagnosis en_US
dc.subject.mesh Immunoglobulin E/ blood en_US
dc.subject.mesh Male en_US
dc.subject.mesh Middle Aged en_US
dc.subject.mesh Physician's Practice Patterns/statistics & numerical data en_US
dc.subject.mesh Physicians, Family/psychology/ statistics & numerical data en_US
dc.subject.mesh Questionnaires en_US
dc.subject.mesh Referral and Consultation en_US
dc.subject.mesh Regression Analysis en_US
dc.subject.mesh Reproducibility of Results en_US
dc.subject.mesh Southeastern United States en_US
dc.title Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes en_US
dc.type article en_US
dc.date.captured 2009-04-27 en_US
dc.identifier.doi doi:10.1186/1471-230X-6-16 en_US
dc.identifier.pmid 18727827 en_US

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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